Acridine sulfonamide and its derivatives are highly sensitive chemiluminescent labels that are widely used in clinical diagnostics. Typically, N-hydroxysuccinimide (NHS) ester of an acridine sulfonamide derivative react with primary amines of target protein in slightly alkaline conditions to form stable amide bonds. After the reaction, the free N-hydroxysuccinimide is removed by dialysis or desalting. In the presence of alkaline peroxide, the acridine-labeled protein emits light independently, without the need for an enzyme catalyst.
Pre-Trigger & Trigger Solution For Acridinium Chemiluminescence System
For use as pre-trigger & trigger solution in acridinium chemiluminescence immunoassay.
Shelf life and storage, 18 months shelf life at 2-8°C.
Substrates
Substrates
Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates in ELISA assay. This substrate develops soluble blue products and can be read spectrophotometrically at 370 or 655 nm. Acid can be added to stop th
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